![]() We filtered the supernatants with a 0.4 μm polycarbonate filter. We shook samples for 8 h and then centrifuged them at 3,700 rpm for 15 min. This step produces effervescence, so the solution should be added slowly to the sample. 147.12 g/mol 7.4 g) was added to each sample split, followed by 200 mL of citrate-bicarbonate solution (pH 7.6). For a given sample, we weighed four sample replicates (2 g) and placed each in 250 mL HDPE bottles. ![]() ![]() Prior to the extraction, we freeze-dried, ground and sieved sediment samples to less than 125 μm (Ruttenberg 1992). These procedures apply to all NMR spectra files. ![]()
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